Loratory equipment

Low-resolution EI (electron ionization) is used to measure the spectra of synthetic and natural organic substances. We will measure the spectra for you on a GCMS-QP2010 (Shimadzu) instrument with a quadrupole mass filter in the range of 15 to 1000 Da, either by direct input DIP (direct inlet probe), or we will pre-separate the mixtures of volatile substances on a gas chromatograph with split/splitless injection. We have a full range of chromatographic columns with different stationary phases. We can offer CI (chemical ionization) in positive and negative modes in special cases.

Among other ionization techniques, we offer electrospray ionization (ESI), atmospheric pressure chemical ionization (APCI), and atmospheric pressure photoionization (APPI) for polar less volatile substances. These methods provide both positive and negative ions. We will measure your spectra on an amaZon SL mass spectrometer (installation 2018) or Esquire 3000 from Bruker with an ion trap that allows you to measure up to MS11 in the range of 50 to 6000 Da for singly charged ions. This mass spectrometer is connected online to an HP 1100 HPLC system, allowing separation by gradient elution in both normal and reverse phases using a DAD detector.

The laboratory is also equipped with a MALDI TOF/TOF Ultraflex device (Bruker), which allows us to analyze large molecules or substances that are difficult to ionize with previous ionization techniques. Thus, we cover a wide spectrum of substances, allowing us to measure spectra in low to medium resolution. The maximum resolving power (RP) we can achieve is 30,000 if the reflectron is installed on the Ultraflex device.                                                                         

The other equipment included a mass spectrometer based on a hybrid Q-TOF arrangement (Bruker) with a selection quadrupole, a collision cell, and TOF detection with a single reflection. The system uses an ion source based on atmospheric pressure electrospray (ESI) and allows the connection of capillary electrophoresis. The device can be used for accurate mass measurement with a resolution higher than 23,000 FWHM in the total mass interval of 20 - 40,000 m/z in both positive and negative modes.

The date of installation 2016

The Agilent 7100 (CE/MS) Capillary Electrophoresis offers rapid separation with excellent efficiency and resolution that (for some analytes) is unattainable by liquid chromatography. It can be used independently, especially with the HRMS mass detector.

The date of installation 2018